Mastitis; Bovine milk; LIBS; CN and C-2;BOVINE-MILK; DAIRY-COWS; SPECTROSCOPY; DIAGNOSIS

The present work focuses on the possibility of using the evolution of the spectral molecular bands of cyanide (CN) and carbon (C-2) in the laser-inducedbreakdown spectra of cow's milk for mastitis characterization. It has been found that the intensities of these molecular bands are directly proportional to the mastitis symptoms and consequently to the somatic cells count (SCC). The results obtained using laser-induced breakdown spectroscopy (LIBS) technique in the present measurements proved that it is a direct, straightforward, and easy method for discrimination between mastitis milk and normal milk. A linear relation between the CN and C-2 spectral line intensities and the corresponding average of the SCC has been obtained. Such linear relationship can be easily exploited in the identification and characterization of mastitic milk samples.

Laser-induced breakdown spectroscopy; melanocyte; skin; pigmentation; nevus;MELANIN SYNTHESIS; NEVUS CELLS; TISSUE; PH; DIFFERENTIATION; ULTRAVIOLET; MELANOSOMES; TYROSINASE; MECHANISM; EXCHANGER

The concentration difference of major elements in melanocytic skin with respect to pigmentation level is analysed by laser-induced breakdown spectroscopy(LIBS) to investigate the applicability of LIBS as an in situ feedback tool for selective and complete laser removal of melanocytic skin tissue like nevus. The skin of black silkie chicken which had a characteristic darkly pigmented perifollicular skin surrounded by lightly pigmented extrafollicular skin was used as the sample. The results showed higher LIBS signal intensities of Ca2+ and Mg2+ but lower intensities of Na+, Cl- and K+ in the perifollicular skin than in the extrafollicular skin, which demonstrated the feasibility to use LIBS as a reliable method to distinguish skin tissues with difference in pigmentation level.