optical demodulation; suspension array; laser-induced breakdown spectroscopy;INDUCED BREAKDOWN SPECTROSCOPY; LASER-INDUCED PLASMA; FLUORESCENCE; IMMUNOASSAYS; MICROBEADS; PROTEINS; LIQUID

A laser-induced breakdown spectroscopy and fluorescence spectroscopy-coupled optical system is reported to demodulate digitally encoded suspension array in fluoroimmunoassay. It takes advantage of the plasma emissions of assembled elemental materials to digitally decode the suspension array, providing a more stable and accurate recognition to target biomolecules. By separating the decoding procedure of suspension array and adsorption quantity calculation of biomolecules into two independent channels, the cross talk between decoding and label signals in traditional methods had been successfully avoided, which promoted the accuracy of both processes and realized more sensitive quantitative detection of target biomolecules. We carried a multiplexed detection of several types of anti-IgG to verify the quantitative analysis performance of the system. A limit of detection of 1.48 x 10(-10) M was achieved, demonstrating the detection sensitivity of the optical demodulation system. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.

INDUCED BREAKDOWN SPECTROSCOPY; QUANTUM DOTS; MICROSPHERE IMMUNOASSAY; COATINGS; BARCODES

We provided a microbeads preparation method with reversed-phase microemulsion and ultraviolet light curing for laser induced breakdown spectroscopybased digital encoding suspension array. By combining reversed-phase microemulsion and ultraviolet light curing together, we created an one-step system for preparation of microbeads. The stability and universality of this method had been confirmed in preparation of microbeads with different encoding materials. With further experiments in surface modification and multiplexed detection, we proved that the synthesized microbeads were available to prepare suspension array and could provide accurate encoding performance in multiplexing.